Molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from Hevea brasiliensis.
Identifieur interne : 000B74 ( Main/Exploration ); précédent : 000B73; suivant : 000B75Molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from Hevea brasiliensis.
Auteurs : Orawan Bunyatang [Thaïlande] ; Nion Chirapongsatonkul [Thaïlande] ; Phuwadol Bangrak [Thaïlande] ; Robert Henry [Australie] ; Nunta Churngchow [Thaïlande]Source :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2016.
Descripteurs français
- KwdFr :
- Antienzymes (composition chimique), Antienzymes (métabolisme), Antienzymes (pharmacologie), Clonage moléculaire (MeSH), Hevea (composition chimique), Hevea (génétique), Hevea (métabolisme), Mycelium (croissance et développement), Phytophthora (croissance et développement), Protéines recombinantes (composition chimique), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Protéines recombinantes (pharmacologie), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéines végétales (pharmacologie).
- MESH :
- composition chimique : Antienzymes, Hevea, Protéines recombinantes, Protéines végétales.
- croissance et développement : Mycelium, Phytophthora.
- génétique : Hevea, Protéines recombinantes, Protéines végétales.
- métabolisme : Antienzymes, Hevea, Protéines recombinantes, Protéines végétales.
- pharmacologie : Antienzymes, Protéines recombinantes, Protéines végétales.
- Clonage moléculaire.
English descriptors
- KwdEn :
- Cloning, Molecular (MeSH), Enzyme Inhibitors (chemistry), Enzyme Inhibitors (metabolism), Enzyme Inhibitors (pharmacology), Hevea (chemistry), Hevea (genetics), Hevea (metabolism), Mycelium (growth & development), Phytophthora (growth & development), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Proteins (pharmacology), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Recombinant Proteins (pharmacology).
- MESH :
- chemical , chemistry : Enzyme Inhibitors, Plant Proteins, Recombinant Proteins.
- chemical , genetics : Plant Proteins, Recombinant Proteins.
- chemical , metabolism : Enzyme Inhibitors, Plant Proteins, Recombinant Proteins.
- chemical , pharmacology : Enzyme Inhibitors, Plant Proteins, Recombinant Proteins.
- chemistry : Hevea.
- genetics : Hevea.
- growth & development : Mycelium, Phytophthora.
- metabolism : Hevea.
- Cloning, Molecular.
Abstract
A novel cDNA encoding a bi-functional α-amylase/subtilisin inhibitor (HbASI) was isolated from rubber (Hevea brasiliensis) leaves cultivar RRIM600. The HbASI had strong homology with the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. Its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from Ricinus communis (72% identity). Genomic sequencing indicated that the HbASI gene contained no introns. The messenger RNA of HbASI was detected in leaf, hypocotyl and root. The recombinant HbASI expressed extracellularly in Pichia pastoris exhibited inhibitory activity against α-amylase from Aspergillus oryzae, trypsin and subtilisin A. The HbASI gene was induced in the rubber leaves infected with a rubber tree pathogen, Phytophthora palmivora. It was also enhanced by salicylic acid (SA) treatment and mechanical wounding. In addition, the biological activity of the HbASI protein involving in the plant defence responses was also investigated. The HbASI at a concentration of 0.16 mg mL(-1) could inhibit the mycelium growth of P. palmivora. These data suggested that the HbASI protein might play a crucial role in defence against pathogen of rubber trees.
DOI: 10.1016/j.plaphy.2016.01.014
PubMed: 26854410
Affiliations:
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Electronic address: nunta.c@psu.ac.th.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112</wicri:regionArea><wicri:noRegion>Songkhla 90112</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular (MeSH)</term><term>Enzyme Inhibitors (chemistry)</term><term>Enzyme Inhibitors (metabolism)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Hevea (chemistry)</term><term>Hevea (genetics)</term><term>Hevea (metabolism)</term><term>Mycelium (growth & development)</term><term>Phytophthora (growth & development)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Proteins (pharmacology)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Recombinant Proteins (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antienzymes (composition chimique)</term><term>Antienzymes (métabolisme)</term><term>Antienzymes (pharmacologie)</term><term>Clonage moléculaire (MeSH)</term><term>Hevea (composition chimique)</term><term>Hevea (génétique)</term><term>Hevea (métabolisme)</term><term>Mycelium (croissance et développement)</term><term>Phytophthora (croissance et développement)</term><term>Protéines recombinantes (composition chimique)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines recombinantes (pharmacologie)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Protéines végétales (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Enzyme Inhibitors</term><term>Plant Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Enzyme Inhibitors</term><term>Plant Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term><term>Plant Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Hevea</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antienzymes</term><term>Hevea</term><term>Protéines recombinantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Mycelium</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hevea</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Mycelium</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hevea</term><term>Protéines recombinantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hevea</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antienzymes</term><term>Hevea</term><term>Protéines recombinantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term><term>Protéines recombinantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel cDNA encoding a bi-functional α-amylase/subtilisin inhibitor (HbASI) was isolated from rubber (Hevea brasiliensis) leaves cultivar RRIM600. The HbASI had strong homology with the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. Its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from Ricinus communis (72% identity). Genomic sequencing indicated that the HbASI gene contained no introns. The messenger RNA of HbASI was detected in leaf, hypocotyl and root. The recombinant HbASI expressed extracellularly in Pichia pastoris exhibited inhibitory activity against α-amylase from Aspergillus oryzae, trypsin and subtilisin A. The HbASI gene was induced in the rubber leaves infected with a rubber tree pathogen, Phytophthora palmivora. It was also enhanced by salicylic acid (SA) treatment and mechanical wounding. In addition, the biological activity of the HbASI protein involving in the plant defence responses was also investigated. The HbASI at a concentration of 0.16 mg mL(-1) could inhibit the mycelium growth of P. palmivora. These data suggested that the HbASI protein might play a crucial role in defence against pathogen of rubber trees. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26854410</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>101</Volume><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from Hevea brasiliensis.</ArticleTitle><Pagination><MedlinePgn>76-87</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(16)30013-4</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2016.01.014</ELocationID><Abstract><AbstractText>A novel cDNA encoding a bi-functional α-amylase/subtilisin inhibitor (HbASI) was isolated from rubber (Hevea brasiliensis) leaves cultivar RRIM600. The HbASI had strong homology with the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. Its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from Ricinus communis (72% identity). Genomic sequencing indicated that the HbASI gene contained no introns. The messenger RNA of HbASI was detected in leaf, hypocotyl and root. The recombinant HbASI expressed extracellularly in Pichia pastoris exhibited inhibitory activity against α-amylase from Aspergillus oryzae, trypsin and subtilisin A. The HbASI gene was induced in the rubber leaves infected with a rubber tree pathogen, Phytophthora palmivora. It was also enhanced by salicylic acid (SA) treatment and mechanical wounding. In addition, the biological activity of the HbASI protein involving in the plant defence responses was also investigated. The HbASI at a concentration of 0.16 mg mL(-1) could inhibit the mycelium growth of P. palmivora. These data suggested that the HbASI protein might play a crucial role in defence against pathogen of rubber trees. </AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bunyatang</LastName><ForeName>Orawan</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chirapongsatonkul</LastName><ForeName>Nion</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bangrak</LastName><ForeName>Phuwadol</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>School of Science, Walailak University, 222, Thasala, Nakhon Si Thammarat 80160, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henry</LastName><ForeName>Robert</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Queensland Alliance for Agriculture and Food Innovation, University of Queensland, 306 Carmody Road, St Lucia, QLD 4072, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Churngchow</LastName><ForeName>Nunta</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. Electronic address: nunta.c@psu.ac.th.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>01</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="Y">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028482" MajorTopicYN="Y">Hevea</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="Y">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Defence responses</Keyword><Keyword MajorTopicYN="N">Hevea brasiliensis</Keyword><Keyword MajorTopicYN="N">Phytophthora palmivora</Keyword><Keyword MajorTopicYN="N">Protease inhibitors</Keyword><Keyword MajorTopicYN="N">α-Amylase/subtilisin inhibitor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>12</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26854410</ArticleId><ArticleId IdType="pii">S0981-9428(16)30013-4</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2016.01.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Bunyatang, Orawan" sort="Bunyatang, Orawan" uniqKey="Bunyatang O" first="Orawan" last="Bunyatang">Orawan Bunyatang</name></noRegion><name sortKey="Bangrak, Phuwadol" sort="Bangrak, Phuwadol" uniqKey="Bangrak P" first="Phuwadol" last="Bangrak">Phuwadol Bangrak</name><name sortKey="Chirapongsatonkul, Nion" sort="Chirapongsatonkul, Nion" uniqKey="Chirapongsatonkul N" first="Nion" last="Chirapongsatonkul">Nion Chirapongsatonkul</name><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name></country><country name="Australie"><noRegion><name sortKey="Henry, Robert" sort="Henry, Robert" uniqKey="Henry R" first="Robert" last="Henry">Robert Henry</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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